Eureka, we are back to spoilage yeasts. Today, I would like to introduce another spoilage yeast called Kluyveromyces marxianus (anamorph Candida kefyr). A yeast first described by EC Hansen in 1888 and named Saccharomyces marxianus after Marx, the person who originally isolated K. marxianus from grapes [Fonseca et al, 2008].
Where do I work?
K. marxianus can be isolated from dairy products, kefir, yoghurt, fermented milk, pozol (Mexican fermented corn dough), sorghum beer, cheese, prickly pear, decaying plants and insects. And a study reveiled, that K. marxianus is even involved in coffee fermentation [Jeong H et al, 2012; Kurtzman et al, 2011; Masoud et al, 2004; Vieira-Dalode G et al, 2007].
What about beer?
I could not find a study where K. marxianus could be identified/isolated from barley based beers. Nevertheless, there is more than just barley based beers. I would like to quickly discuss another malt based beverage called gowé made in Bénin, West Africa. This beverage is available as a cooked paste which gets diluted with milk and water leading to a sweet beverage [Vieira-Dalodé et al, 2007]. Never tried gowé myself and honestly haven’t heard about it before either.
A study to identify micro-organisms involved in the production of sorghum gowé identified K. marxianus as a dominant yeast species [Vieira-Dalodé et al, 2007]. Gowé is made from malted sorghum (Sorghum bicolour) according to the work flow shown in Fig 1. The sorghum grains are cleaned and divided into two parts. 25% of the grains get soaked, drained and left for germination before sun dried. A process very similar to the malting process used to make barley malt. The malted sorghum gets milled and kneaded with water. This mixture is left for a primary fermentation. The 75% part of sorghum is left nonmalted and 15% is combined with hot water to form a slurry which is re-combined with the remaining 60% of the nonmalted sorghum and the fermenting dough to form a mixture with a temperature of about 50-60°C. This mixture is left for a secondary fermentation resulting in gowé.
Fig 1: Production of gowé. Figure taken from Vieira-Dalodé et al, 2007
Vieira-Dalodé et al took samples during primary and secondary fermentation to identify the lactic acid bacteria and yeasts involved in the fermentation of gowé. The authors could identify K. marxianus, Pichia anomala, Candida krusei and Candida tropicalis during the fermentation. K. marxianus as well as P. anomala were present from an early stage on and the most important species during the first hours of primary fermentation. C. krusei and C. tropicalis peaked after 12 h.
What is so special about me?
K. marxianus has several biotechnological applications and is used to produce beta-galactosidase, inulinase or pectinase [Jeong et al, 2012].. Since this is yet another yeast with biotechnological applications, its genome was sequenced in 2012 by Jeong et al. The authors sequenced strain KCTC 17555 using Illumina Genome Analyzer IIx and assembled a 10.9 Mb genome allocated into eight chromosomal groups. Of 4,998 predicted proteins, 91% were also present in Kluyveromyces lactis. Furthermore, key enzymes for xylose assimilation are also present (as previously discussed in Pichia kudriavzevii) suggesting this yeast can be used for biofuel production as well [Jeong et al, 2012].
Where can you find me?
As K. marxianus is present in dairy products and many other sources, it is very likely one could pick up this yeast from these sources. The challenging part would be to identify K. marxianus from all the possibly isolated yeasts.
Some biochemical stats about me for yeast ranchers
Data summarized from Kurtzman et al (2011).
| Systematic name: | Kluyceromyces marxianus (anamorph Candida kefyr) | |
| Synonyms: | There are a lots of accepted synonyms for this yeasts. Just some examples: Saccharomyces marxianus, Kluyveromyces bulgaricus, Hansenula pozolis |
|
| Growth on YM agar: | Cell morphology: | Ellipsoidal to cylindrical, 2-6 µm x 3-11 µm |
| Clustering: | Occurring as single cells, pairs or short chains | |
| Pseudohyphae: | Observed | |
| Pellicle formation: | May form a thin pellicle | |
| Fermentation: | Glucose: | Positive |
| Galactose: | Weak | |
| Sucrose: | Positive | |
| Maltose: | Negative | |
| Lactose: | Variable | |
| Raffinose: | Positive | |
| Trehalose: | Negative | |
Since K. marxianus is negative for maltose fermentation, it is very unlikely that a single K. marxianus beer fermentation would work (as mainly maltose is present in wort). So far for the theory. If anyone out there silly enough to try this yeast for a beer fermentation, please let me know. Over and out.
Bibliography
- Fonseca GG, Heinzle E, Wittmann C, Gombert AK (2008) The yeast Kluyveromyces marxianus and its biotechnological potential. Appl Microbiol Biotechnol, Vol 79(3), p 339-54
- Jeong H, Lee DH, Kim SH, Kim HJ, Lee K, Song JY, Kim BK, Sung BH, Park JC, Sohn JH, Koo HM, Kim JF (2012) Genome sequence of the thermotolerant yeast Kluyveromyces marxianus var. marxianus KCTC 17555. Eukaryot Cell, Vol 11(12):1584-5. doi: 10.1128/EC.00260-12, http://www.ncbi.nlm.nih.gov/pubmed/23193140
- Kurtzman CP, Fell JW, Boekhout T (2011) The Yeasts, a Taxonomic Study. Volume 1. Fifth edition. Elsevier (Link to sciencedirect)
- Masoud W, Cesar LB, Jespersen L, Jakobsen M (2004) Yeast involved in fermentation of Coffea arabica in East Africa determined by genotyping and by direct denaturating gradient gel electrophoresis, Yeast, Vol 21(7), p 549-56
- Vieira-Dalode G, Jespersen L , Hounhouigan J, Moller PL, Nago CM, Jakobsen M (2007) Lactic acid bacteria and yeasts associated with gowé production from sorghum in Bénin, Journal of Applied Microbiology, Vol 103, p 342–349
Eureka Brewing 
Love this series, I’ve been mining for info on these non-sacch strains and it’s definitely few and far between. There’s a strain of Kluyveromyces marketed to wine makers by CHR Hansen:
“CONCERTO: launched in 2012, this pure Kluyveromyces thermotolerans strain is ideal for wines produced in warm climate regions. It produces lactic acid giving roundness and balanced acidity to the wines and therefore improving flavour complexity. Definitively a product to use before you inoculate your usual yeast if you produce red or white wines in a warm to hot area.”
They also sell a Pichia kluyveri strain and a T. Delbrueckii strain, all under their Vinflora line. I’ve been wanting to order them, but they’re pretty pricey. Feel free to PM me if you want to split a brick 🙂
Even more interesting is this Metschnikowia pulcherrima strain sold as FLAVIA MP346 from Lallemand.
Thanks for reading. Concerto sounds like an interesting strain to try on a batch of beer.
Cool topic! I just came across this description about the use of Kluyveromoyces genus,et.al., as lactose fermenting yeasts (in the book, “On Food and Cooking: The Science and Lore of the Kitchen” by Harold Mcgee): “For thousands of years, the nomads of central Asia have made Koumiss from mares’s milk…[a] tart, effervescent drink, with 1 to 2% alcohol and 0.5 to 1% acid, remains very popular in Russia.”
I coudn;t help but wonder what the addition of anyone of the species of the genus to a milk stout might do!?
That would for sure be dependent on the genus used. If one uses a genus which is capable of fermenting lactose, one would expect to see the Kluyceromyces fermenting the lactose away, right?
I ended up reading this article because I just bought a bottle from Guineu brewery in Spain called “No Sucks” that says it is 100% Kluyveromyces. It’s 3.3% abv. I haven’t opened it yet. Just thought you’d like to know someone was silly enough to try it.
Thanks for sharing. I know about the existence of said beer but never tried it myself. I am not sure what kind of Kluyveromyces they used for this one. K. lactis seems very likely to me or K. thermotolerans (which I am going to try myself in 2015).
Sam,
Have you talked with Matt – https://matthumbard.wordpress.com/?page_id=1?replytocom=277#respond . He has been doing some trials with it and was even generous enough to offer to send some to me once he has some bottled.
Hi Jeff, I haven’t talked to Matt about this yet. I however am in contact with a local Swiss brewer that is interested in projects like this. If we agree to brew a beer, it might even turn up in the US at some point 🙂
I’m trying to make milk beer from this yeast, can you give me some advice? Thks bro
Really sorry but I mever made milk beer nor brewed with K. marxianus before. Therefore cannot help you out here.
How did you get on with your milk beer trails? I’m in the process or researching for a whey based brew and interested if it’s worked for you?
Cheers James
Hiya, I actually never went on playing around with whey nor milk based beverages. Just don’t find the time. Whey should work since there is at least one brewery in Austria doing a whey beer that I know of.
Cheers Sam