Eureka, today ends the pentade about agar plates with a short post about kombucha. The other posts can be found here. I plated some kombucha on a Sabouraud agar plate and incubated it for three days. Here is what I got.
I could observe at least three different colonies:
– Off-white color, even, circular, glossy, convex, 2 mm diameter.
– Transparent, circular, less than 1 mm diameter
– wavy, white, 1 mm diameter
I expect the off-white colonies to be yeasts. The other colonies must be bacteria of some kind. The plate has a distinct vinegar smell. My microscopy analysis revealed that the off-white colonies were again typical Saccharomyces cerevisia yeast cells. And the other colonies are bacteria of different shapes. I did not take any photographs due to lack of time. I am very sorry about that.
Lets summarize, there are several bacteria strains in kombucha as well as yeasts. I am not that surprised about the presence of bacteria as the kombucha had a vinegary smell as I tasted it. There should be some Acetobacter in there I guess. And some other bacteria as well. As well as yeast(s). Can’t tell if there are more than one strain(s).
Well, this is it. The pentade is over. I had a lot of fun with the different bugs and learned a lot of new stuff concerning yeasts and bacteria. I hope you enjoyed it as well. I will post a summary about the different plates by the end of February 2012 with new results as well.
What’s my next project concerning wild bugs? I haven’t planned a new experiment yet but I might try out the potato agar mentioned by BKYeast. If you do not already know BKYeast’s page, please check it out. There are some pretty neat results and pictures of different bugs. Cheers to everyone!