Time for another experiment. After brewing some wheat beers I asked myself, what could do a different yeast to a wheat beer flavor-wise. To determine the impact of different yeasts I started a little experiment. I will post the results in a separate post as this experimental design description is quite long.
Aim of the project
The goal of this project was to determine the flavor impact of different wheat yeast strains. Further on, has the dry yeast WB-06 similar/the same flavors as the Wyeast #3068 Weihenstephan? (some people suggest that WB-06 is the same strain as Wyeast use for their #3068)
To get an idea of the flavor profile of the used wheat strains, it is inevitable to research for some information about the yeast strains. The following information are from the yeast suppliers Wyeast, Danstar and Fermentis:
#3068 Weihenstephan: 73- 77% (18- 24°C) (64- 75°F)
Banana ester, phenols and clove-like characteristics. Alcohol Tolerance: 10% ABV (alcohol by volume), low flocculation.
#3056 Bavarian Wheat Blend: 73- 77% (18- 23°C) (64- 73°F)
Blend of top-fermenting ale an wheat strains producing mildly estery and phenolic wheat beers. Alcohol Tolerance: 10% ABV, medium flocculation.
#3333 German Wheat: 73- 76% (17- 24°C) (63- 75°F)
Unique sharp tart crispness, fruity, sherry-like palate. Alcohol Tolerance: 10% ABV, high flocculation. Banana and clove. It seems that Wyeast changed their homepage since I planned my experiment.
#3638 Bavarian Wheat: 70- 76% (18- 24°C) (64- 75°F)
Complex flavor and aroma. Balance of banana and bubble gum esters with litchi and apple/plum esters and clove. Alcohol Tolerance: 10% ABV, low flocculation.
#1010 American Wheat: 74- 78% (14- 23°C) (57- 73°F)
Dry fermenting, slightly tart, crisp beer. Ideal for beers where low ester profile is desirable. Alcohol Tolerance: 10% ABV, low flocculation.
#3942 Belgian Wheat: 72- 76% (18-23°C) (64- 73°F)
Moderate esters and minimal phenolic. Apple, bubblegum and plum-like aromas blend nicely with malt and hops. This strain will finish dry with a hint of tartness. Alcohol tolerance: 12% ABV, medium flocculation.
Coopers Wheat: (19- 21°C) (66- 70°F)
Isolated from a Coppers Wheat kit. It might be no real wheat yeast.
Dry yeast Fermentis Safbrew WB-06: (15- 24°C) (59- 75°F)
Subtle ester and phenol flavor notes. Low sedimentation. Final gravity: high.
Dry yeast Danstar Munich: (> 17°C) (> 63°F)
Medium to high attenuation, non flocculent strain, esters to both palate and nose with typical banana notes.
Isolated from a local wheat beer (Weizen, Burgdorfer Gasthausbrauerei)
Wheat beer profile
BJCP: 15A Weizen/Weissbier, OG: 1.044-1.052, FG: 1.010-1.014, IBU: 8-15, SRM: 2-8, ABV: 4.3- 5.6%.
Methods: Resting at 44°C (111°F) enhances the 4-vinylguajacol production (precursor for the wheat typical clove aroma). Typical pitching rates for wheat beers: 5- 10 E6 cells mL-1.
|#3068 Weihenstephan||X||X||X||X||Typical wheat aromas|
|#3056 Bavarian Blend||X||X||No banana|
|#3333 German Wheat||X||X||X||No banana|
|#3638 Bavarian Wheat||X||X||X|
|#1010 American Wheat||X||No typical European wheat aromas|
|3942 Belgian Wheat||X||X||Fruity character, light phenolic notes|
|WB-06||X||X||X||No banana, phenolic|
|Danstar Munich||X||X||X||Typical wheat yeast, comparable to #3638|
|Burgdorfer Weizen||No idea|
|Numbers:||Volume||20 L (5.3 gal)
|Terminal gravity||3.5 – 4.2°P (yeast dependent)|
|Color||Around 4 EBC|
|ABV||Around 5.3 %|
|Grains:||Pilsner malt (3 EBC)||2.1 kg|
|Wheatmalt (3 EBC)||2.6 kg|
|Acidified malt (5 EBC)||0.1 kg (see rest)|
|Hops:||Hallertauer (4.2% AA)||11.4 g and boil for 60 min11.4 g and boil for 30 min|
|Water:||Burgdorf||Mash: 12 L (3.2 gal), lauter: 19 L @78°C (174°F) (5 gal)
|Rest:||4 steps||Mash in @47°C, 20 min @ 45°C (113°F), 15 min @53°C (128°F)(add acidified malt), 30 min @63°C (145°F), 40 min @72°C (162°F), mash out @ 78°C|
|Boil:||Total 60 min|
|Maturation:||Carbonation (CO2 vol)||2 vol for bottles|
|Maturation time||3 Weeks @18°C (64°F)
Used for each yeast two times 2 L (0.5 gal) of wort. Inoculated the yeasts at 12-15°C (54- 59°F) and primary fermentation at 18- 22°C (64- 72°F) till final gravity reached (one week). Pitching rate: 5 E6 cells mL-1. Secondary fermentation in bottles for one week at 20°C (68°F). Maturation at 4°C (39°F) for three weeks.
To ferment ten batches simultaneously I had to use small buckets. They have a volume of about 2.5 Liters (0.66 gal).
As I had some strains in my library, I had to cultivate some strains from my library and some from Propagators from Wyeast. The strains from my library: Inoculated yeasts to 200 mL (0.05 gal) of 10°P sterile wort (20 g of dried malt extract to 200 mL tap water) and let them ferment on my stirrer. I propagated the #3068, #3056, Coopers Wheat and Burgdorfer Weizen as previously mentioned.
For the Propagator I used 20 mL of the volume of the packages. And for the dry yeasts: 1.25 g of WB-06 to 2 liters, and 2.5 g of Danstar Munich to 2 liters (0.5 gal) of wort.
I determined the cell densities of all the starters (expect the Propagators) by use of a Neubauer cell count device to ensure the right pitching rates.
Three batches (3 times 20 L (5 gal)) were made to supply enough wort for the experiment:
1. Batch (20 Liters) for #3068 Weihenstephan (reference), WB-06, #1010 American Wheat, #3333 German Wheat and Coopers.
2. Batch (20 Liters) for #3068 Weihenstephan (reference), #3056 Bavarian Wheat Blend, Burgdorfer Weizen, #3638 Bavarian Wheat.
3. Batch (40 Liters) for #3068 Weihenstephan (reference), #3942 Belgian Wheat. The rest of the volume was used for a different beer and had nothing to do with this experiment.
The reference strain (#3068 Weihenstephan) was used to ensure the comparability of the three batches. Each yeast was inoculated to two different worts as a repeat.
Determine the differences between the repeats of the same yeast. Then determine differences between the batches with the reference strain. Determine differences between the WB-06 strain and the #3068 Weihenstephan strain. Define flavor profiles of the different yeasts. Which is the most appropriate strain to use in a wheat beer according to the tasting panels opinion?
Results and Discussion
Will be published in a post in the near future as I am still working on the data analysis.